![]() In addition to these primary TSS, this analysis revealed complex structure of transcriptional units such as alternative and internal promoters, potential RNA processing events and 5′ untranslated regions. By 5′-end RNA-seq approach, we mapped more than 1000 TSS upstream of genes. difficile genome resulting from the identification of transcriptional start sites (TSS), promoter motifs and operon structures. We present here the first transcriptional map of the C. difficile 630, 22 genes encoding sigma factors are present suggesting a complex pattern of transcription in this bacterium. difficile promoter structure is still missing. Regulatory pathways underlying the adaptive responses remain understudied and the global view of C. The emerging human enteropathogen Clostridioides difficile is the main cause of diarrhea associated with antibiotherapy. ![]() 5′-end RNA-seq data show 51-bp reads matching to the 5′-transcript ends, while RNA-seq data show reads covering whole transcript. The TSS corresponds to a position with significantly greater number of reads in TAP+ sample, potential cleavage site corresponds to position with large number of reads in both TAP- and TAP+ samples. Sigma factor consensus associated with a given TSS is indicated. The TSS identified by 5′-end RNA-seq are indicated by red broken arrows and potential processing sites are indicated by scissors mark. The 5′-end RNA-seq data for either positive “strand +” or negative “strand −” strands are presented in the panels. On a RNA-seq and 5′-end RNA-seq sequence read mapping visualization, coding sequences are indicated by blue arrows. Cov2HTML ( Monot et al., 2014) was used for the visualization. Representative examples of 5′-end RNA-seq (TAP−/TAP+ profile comparison) and RNA-seq data for dual tandem TSS and internal TSS inside the coding sequences are shown in panels (A,B), respectively. FIGURE S5: TSS mapping of dual (A) and internal (B) promoters.
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